| 1. | Surveys on mycoplasma pulmonis infection of laboratory animals in lanzhou and isolation and identification of the positive strains
兰州地区实验动物肺支原体感染调查和分离鉴定 |
| 2. | The cluster analysis on 126 phenotypic characteristics of 117strains isolated from fermented foods were proceeded , 110 strains are gram positive strains and 7 strains are reference strains as comparison
从新分离的和原来保藏的菌株中，选择了革兰氏阳性菌株110株和7株参比菌株，进行了菌株表型特性的测定。 |
| 3. | The results show that there are 7 strains hpi positive except 1 strain hpi negative . hpi in 6 strains of 7 hpi - positive strains is inserted into asnt - trna site . the expression of fyua gene , which encoded fyua , the receptor of ybt , was upregulated by extracellular ybt level
研究结果提示，除1株eaggec中国分离株hpi ”外，其余7株均为hpi且7株中有6株携带的hpi毒力岛均插入在染色体的asnttrna位点。 |
| 4. | Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly . 7 eaggec hpi - positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi - negative did not so
3n ） ，将待测eaggec菌株的培养上清加入该报告菌株培养物中，用流式细胞术facs方法检测报告菌株荧光强度的变化情况，间接反映ybt的表达与否。 |
| 5. | The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine . multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol . the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified
转化嗜甲醇酵母获得耐受4mg mlg418的菌株，经sds - page电泳和westernblotting检测表明其上清培养液中的蛋白可与猴抗pzp3抗体和兔抗hcg抗体发生特异性的反应。 |
| 6. | The results suggested that eaggec hpi - positive strains expressed the ybt system , but the presence of hpi core part does n ' t mean ybt expression . 2 . research on the function of ybtp , ybtq and ybtx genes in eaggec 17 - 2 ybtp , ybtq and ybtx genes from yen wa were cloned respectively and mutated , then inserted by a selectable substitution with chloramphenicokcaf ) gene in vitro
携带hpi毒力岛的eaggec17毛ybtp 、 ybtq 、 ybtx基因功能研究克隆了小肠结肠炎耶尔森菌hpi毒力岛一rr7和叩结构基因，在体外进行精确突变后，插入氯霉素抗性基因（ cat ）作为选择性标记。 |
| 7. | The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8 . vectors were amplificated in the e . coli dh5 a and were linearized with bgl ii . the linearized vctors were transformed into host strain gs115 . the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa . the recombinated protein was detected with sds - page and western - blot as before
重组菌用甲醇诱导表达，用dot - elisa的方法筛选到表达量较高的菌株。将筛选出的菌株大量的诱导表达，对表达上清处理后，用sds - page和western - blot进行鉴定。同时，用hiprep16 10heparinff肝素亲和柱对表达蛋白进行了初步的纯化。 |
| 8. | Sds - page and westen - blotting validated the expression of pp24 . the expressed specific band was excised from the gel and injected into mice once two weeks for 5 times , then we collected the antiserum from the mice and used it for ifa with chicken embryonic fibroblasts ( cef ) infected by mdv mdll , cvi988 and ga strains respectively . the positive straining was found in the mdv plaques , which shows the in vitro expressed protein of pp24 has some epitopes of mdv
将型mdvmd11株的pp38基因和pp24基因的完整orf分别克隆入真核表达载体pcdna3 . 1 / zeo ( + )中，重组质粒pcdna3 . 1 - pp38和pcdna3 . 1 - pp24在脂质体作用下分别转染cef ，在转染后24 、 48 、 72小时，用单抗h19和pgex - pp24原核表达产物制备的抗血清分别对pcdna3 . 1 - pp38和pcdna3 . 1 - pp24转染的cef进行ifa检测，结果检测到了pp38和pp24在cef中的表达，该试验也使pp24基因在原核和真核系统中的表达得到了相互验证。 |
